How Does Peptide D4E1 Affect the Survival Rate of Soybean Cyst Nematodes?

Supporting files

The purpose of this experiment was to determine the survival rate of Soybean Cyst Nematodes when exposed to peptide D4E1.The null hypothesis is that the peptide will have no effect on the survival rate. The simplified procedure includes extracting SCN from an infested soybean field and counting the number of living nematodes. There were 9 living nematodes in each sample. The extraction process was repeated 8 times so that both the experimental and control groups had 36 living nematodes. After this, the experimental group was exposed to a 200 micromolar solution of peptide D4E1. In contrast, the control group was exposed to distilled water. Both groups were exposed to their solutions for 48 hours. After the 48 hours, both groups were examined underneath a microscope to determine the number of living nematodes post exposure. 5.5% of the peptide exposed group survived, and 97.2% of the water exposed group survived. After constructing confidence intervals, the null hypothesis was rejected. Instead, the data supports the claim that peptide D4E1 decreased the survival rate of Soybean Cyst Nematodes when exposed at 200 micromolar concentration for 48 hours.