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Gel electrophoresis basics

How can we check the success of biotechniques? In what ways can we see if the gene(s) of interest are present?

This unit leads students through the process of setting up chambers, understanding the mechanics and experimenting with various aspects of running gels.

Lessons

Teacher background

DNA fragments (or other macromolecules, such as RNA and proteins) can be separated based on their size and charge. By running a current through a gel containing the molecules of interest, gel electrophoresis causes molecules of different sizes to travel through the gel in different directions or at different speeds, allowing them to be separated from one another. Since DNA molecules have the same amount of charge per mass, gel electrophoresis of DNA fragments separates them based on size only. This allows for observation of many different DNA fragments which might be present in a sample and the sizes relative to one another. Determining the absolute size of a piece of DNA is possible by using a sample of DNA fragments of known sizes.

Overall, biotech jobs are expected to increase by 10% over the coming years. The number of jobs for biotech technicians is expected to be around 88,300 by 2022, up from 80,200 jobs in 2012. To learn more about biotechnology in agriculture, see our Biotechnology carer video. Other careers in biotechnology can be found at biotech-careers.org.

Next gen standards

Science and engineering practices

  • Developing and using models

Crosscutting concepts

  • Cause and effect
  • Systems and system models
  • Structure and function

Disciplinary core ideas/content

  • ESS3C Human impacts on Earth systems
  • LS3A Inheritance of traits
  • LS3B Variations of traits
  • PS2B Types of interactions
  • PS4C Information technologies and instrumentation
  • ETS2 Links among Engineering, technology, science and society
  • ETS2A Interdependence of science, engineering and technology
  • ETS2B Influence of engineering, technology and science on society and the natural world

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